This medium utilizes TOS (Galacto-oligosaccharide) as the primary carbohydrate source, which is highly preferred by Bifidobacterium species. The Peptone from Casein and Yeast Extract provide the essential nitrogen, vitamins, and minerals required for growth. Sodium Propionate acts as a selective agent to suppress the growth of competitive flora. Selectivity is further enhanced by the aseptic addition of the antibiotic Mupirocin. Because Bifidobacteria are obligate anaerobes, the medium is designed for use with anaerobic incubation techniques. The combination of TOS and Mupirocin (TOS-MUP) provides a reliable method for distinguishing bifidobacteria from the high background of other probiotic bacteria often found in dairy formulations.
Advantages
- ISO 29981 Compliance: Specifically designed to meet the international standard for the enumeration of Bifidobacterium in dairy products.
- High Selectivity: The addition of Mupirocin effectively inhibits Lactobacillus acidophilus and other common yogurt starters.
- Optimal Recovery: TOS provides a specialized carbon source that promotes the robust growth of diverse Bifidobacterium strains.
- Standardized Monitoring: Essential for quality control in the production of functional foods and probiotic supplements.
- Clear Results: Allows for accurate counts of bifidobacteria even in the presence of much higher concentrations of other lactic acid bacteria.
Technical specifications
| Catalogue number |
2011 |
| Brand |
Condalab |
| Application |
Enumeration of Bifidobacteria (ISO 29981) |
| Typical Composition (g/L) |
Peptone from Casein (10.0); Yeast Extract (1.0); TOS (10.0); Sodium Propionate (15.0); Potassium Dihydrogen Phosphate (3.0); Dipotassium Phosphate (4.8); Ammonium Sulfate (3.0); Magnesium Sulfate (0.2); L-Cysteine (0.5); Bacteriological Agar (15.0) |
| Final pH (at 25 °C) |
6.3 ± 0.2 |
| Preparation |
62.5 g/L (plus Mupirocin) |
| Storage Temperature |
2 – 25 °C |
Available packaging options
| 2011 |
500 g plastic bottle |
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