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1508 Thioglycollate Fluid Medium EP/USP, Condalab

1508 Condalab

Thioglycollate Fluid Medium (Fluid Thioglycollate Medium) is a versatile liquid medium used for the cultivation of aerobic, anaerobic, and microaerophilic microorganisms. It is primarily designed for sterility testing of pharmaceutical products, biologicals, and medical devices, strictly following the harmonized methods of the European Pharmacopoeia (EP) and the United States Pharmacopeia (USP).

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This medium is formulated to maintain a low oxidation-reduction potential, allowing for the growth of obligate anaerobes without the need for specialized anaerobic jars. The Pancreatic Digest of Casein and Yeast Extract provide nitrogen, vitamins, and minerals. Glucose serves as the energy source. Sodium Thioglycollate and L-Cystine act as reducing agents to deplete oxygen. A small amount of Bacteriological Agar is included to reduce the diffusion of oxygen from the surface into the lower parts of the medium. The redox indicator Resazurin turns pink when oxidized (at the top of the tube) and remains colorless when reduced, providing a visual guide to the anaerobic state of the medium.

Advantages

  • Pharmacopoeia Compliance: Fully compliant with EP, USP, JP, and ISO 7937 standards for sterility testing and microbial detection.
  • Broad Growth Spectrum: Supports the growth of a wide range of bacteria, including fastidious anaerobes, aerobes, and microaerophiles.
  • Internal Anaerobiosis: The combination of reducing agents and agar allows for the testing of anaerobes in standard incubator settings.
  • Visual Quality Control: Resazurin provides an immediate visual indication of whether the medium's anaerobic integrity has been compromised by oxygen exposure.
  • Standardized Testing: Widely used in the pharmaceutical and medical device industries for safety and quality control.

Technical specifications

Catalogue number 1508
Brand Condalab
Application Sterility testing and cultivation of anaerobes (EP/USP)
Typical Composition (g/L) Pancreatic Digest of Casein (15.0); Yeast Extract (5.0); Glucose (5.5); Sodium Chloride (2.5); L-Cystine (0.5); Sodium Thioglycollate (0.5); Resazurin (0.001); Bacteriological Agar (0.75)
Final pH (at 25 °C) 7.1 ± 0.2
Preparation 29.75 g/L
Storage Temperature 2 – 25 °C

Available packaging options

1508 500 g plastic bottle
Brand Condalab
Pack size 500 g