This medium is a modification of Tryptic Soy Agar (TSA) supplemented with Yeast Extract to provide a superior nutrient environment. The Pancreatic Digest of Casein and Soy Peptone provide organic nitrogen, amino acids, and peptides. Yeast Extract is added to provide a high concentration of B-complex vitamins, which are essential for the robust growth of Listeria. Sodium Chloride maintains the osmotic equilibrium, while Dipotassium Phosphate acts as a buffering agent to maintain the pH. TSYEA is non-selective, designed specifically to allow the pathogen to express its natural morphology and purity, ensuring that subsequent tests (such as Gram staining, motility, and the CAMP test) are performed on healthy, characteristic cultures.
Advantages
- ISO 11290 Compliance: Formulated to meet the standardized requirements for the horizontal method of detection and enumeration of Listeria.
- High Nutrient Content: Supplemented with Yeast Extract to support the recovery of stressed Listeria cells from selective media.
- Optimal Morphology: Allows for the clear development of characteristic Listeria colonies, which are small, translucent, and slightly convex.
- Standardized Confirmation: Provides a reliable base for subsequent biochemical identification steps.
- Versatile Application: Can also be used for the maintenance and subculturing of a wide variety of fastidious microorganisms.
Technical specifications
| Catalogue number |
1398 |
| Brand |
Condalab |
| Application |
Confirmation and subculturing of Listeria monocytogenes (ISO 11290) |
| Typical Composition (g/L) |
Pancreatic Digest of Casein (15.0); Soy Peptone (5.0); Yeast Extract (6.0); Sodium Chloride (5.0); Dipotassium Phosphate (2.5); Bacteriological Agar (15.0) |
| Final pH (at 25 °C) |
7.3 ± 0.2 |
| Preparation |
48.5 g/L* *(Note: composition values may vary slightly by batch; follow label instructions) |
| Storage Temperature |
2 – 25 °C |
Available packaging options
| 1398 |
500 g plastic bottle |
