1076 TTC Chapman Agar (Lactose Agar TTC with Tergitol 7), Condalab

This medium is designed to provide rapid presumptive results for coliforms by combining selective inhibition with clear color differentiation. The Peptone Mixture and Beef Extract provide the essential nitrogen, vitamins, and minerals. Lactose is the fermentable carbohydrate. The selectivity is primarily due to Tergitol 7 (Sodium Heptadecyl Sulfate), which strongly inhibits Gram-positive bacteria and limits the swarming of Proteus, allowing for better colony definition. The differentiation is dual-fold: Bromothymol Blue acts as a pH indicator (turning yellow when lactose is fermented), and the addition of TTC (Triphenyltetrazolium Chloride) allows for the identification of organisms that reduce TTC to red formazan. E. coli and coliforms typically appear as yellow colonies with a yellow halo, while non-lactose fermenters remain blue or green.

Advantages

• Highly Selective: Tergitol 7 effectively suppresses the growth of most Gram-positive organisms and prevents the spreading of Proteus species.
• Clear Visual Differentiation: The shift from blue/green to yellow, combined with the reduction of TTC, allows for easy identification of coliforms.
• Optimized for Water Testing: Specifically designed for the membrane filtration method used in monitoring drinking water and environmental water quality.
• Rapid Presumptive Identification: Provides reliable results within 24 hours of incubation.
• Standardized Methodology: Long-standing reliability in laboratories following traditional ISO and European water quality protocols.

Technical specifications

Available packaging options