This medium is specifically designed for applications where the presence of an indicator or a semi-solid agent (agar) might interfere with the interpretation of results or the specific requirements of the testing protocol. The Pancreatic Digest of Casein and Yeast Extract provide essential nitrogen, vitamins, and minerals to support the growth of aerobic, anaerobic, and microaerophilic bacteria. Glucose (Dextrose) serves as the primary energy source. Sodium Thioglycollate and L-Cystine act as reducing agents, lowering the oxidation-reduction potential of the medium to allow for the growth of obligate anaerobes. Because this NIH version is a clear broth without agar, it is ideal for testing samples that already possess some degree of turbidity, ensuring that even slight bacterial growth can be detected.
Advantages
- Indicator-Free: Absence of resazurin makes it suitable for testing products where chemical reactions with the indicator could occur.
- High Clarity: The lack of agar results in a clear liquid, facilitating the detection of microbial growth (turbidity) in samples that are naturally cloudy.
- Versatile Growth Support: Supports the proliferation of a broad spectrum of bacteria, including fastidious anaerobes and aerobes.
- Sterility Testing Optimized: Specifically formulated for the examination of biologicals and pharmaceuticals according to NIH guidelines.
- Effective Reducing Power: Maintains an anaerobic environment in the lower portions of the tube, even without a mechanical seal.
Technical specifications
| Catalogue number |
1241 |
| Brand |
Condalab |
| Application |
Sterility testing of biologicals and cultivation of anaerobes |
| Typical Composition (g/L) |
Pancreatic Digest of Casein (15.0); Yeast Extract (5.0); Glucose (5.0); Sodium Chloride (2.5); Sodium Thioglycollate (0.5); L-Cystine (0.5) |
| Final pH (at 25 °C) |
7.1 ± 0.2 |
| Preparation |
29.0 g/L |
| Storage Temperature |
2 – 25 °C |
Available packaging options
| 1241 |
500 g plastic bottle |
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