TPY Broth provides a complex and rich nutrient environment tailored to the fastidious growth requirements of Bifidobacteria. The combination of Tryptone (Pancreatic Digest of Casein) and Phytone (Papainic Digest of Soy) provides a wide range of nitrogenous compounds, amino acids, and long-chain peptides. Yeast Extract further enriches the medium with essential B-complex vitamins and growth factors. Glucose serves as the primary fermentable carbohydrate and energy source. To support the anaerobic nature of Bifidobacterium, the medium includes L-Cysteine Hydrochloride, which acts as a reducing agent to lower the oxidation-reduction potential. Dipotassium Phosphate and Monopotassium Phosphate provide a buffering system to maintain pH stability, while various salts (Magnesium, Manganese, Zinc, and Calcium) serve as essential cofactors for bacterial enzymes.
Advantages
- Optimized for Probiotics: Specifically formulated to support the robust growth of the Bifidobacterium genus, which is often difficult to culture in standard media.
- Rich Nutrient Profile: The synergistic effect of casein and soy peptones ensures the recovery of even the most demanding strains.
- Anaerobic Support: Inclusion of L-Cysteine helps maintain a reduced environment, protecting cells from oxidative stress during initial growth.
- Stable Environment: Balanced minerals and buffers prevent rapid pH drops that could inhibit bacterial viability.
- Versatile Application: Ideal for preparing inocula for further testing, biomass production, or quality control in dairy and supplement manufacturing.
Technical specifications
| Catalogue number |
1591 |
| Brand |
Condalab |
| Application |
Cultivation and enrichment of Bifidobacterium species |
| Typical Composition (g/L) |
Tryptone (10.0); Phytone (5.0); Yeast Extract (2.5); Glucose (5.0); L-Cysteine HCl (0.5); K2HPO4 (2.0); KH2PO4 (1.0); MgSO4·7H2O (0.5); MnCl2·4H2O (0.34); ZnSO4·7H2O (0.25); CaCl2 (0.15); FeCl3·6H2O (0.1) |
| Final pH (at 25 °C) |
6.5 ± 0.2 |
| Preparation |
41.6 g/L |
| Storage Temperature |
2 – 25 °C |
Available packaging options
| 1591 |
500 g plastic bottle |
