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8045 Agarose D-5, Condalab

8045 Condalab

Agarose D-5 is a high-quality, high-gel strength agarose specifically engineered for the separation of large DNA fragments and for applications requiring exceptional gel robustness. Due to its unique physical properties, it is the preferred choice for pulsed-field gel electrophoresis (PFGE) and for the preparation of agarose beads used in chromatography and protein purification.

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Agarose D-5 is characterized by its high gel strength (≥ 1,800 g/cm² at 1.5%) and high gelation temperature, which provides superior thermal stability and mechanical resistance. It is highly purified to ensure a low electroendosmosis (EEO) value, allowing for the precise migration of nucleic acids with minimal band distortion. This agarose is specifically processed to be free of detectable DNase and RNase activity, ensuring the integrity of sensitive genetic samples during long electrophoresis runs. Its high transparency makes it ideal for clear visualization and documentation of results.

Advantages

  • Exceptional Gel Strength: Allows for the easy handling of low-concentration gels and provides the durability needed for long-duration PFGE runs.
  • Optimized for Large Fragments: Excellent for the separation of DNA molecules larger than 20 kb and up to several megabases.
  • High Purity: Low EEO ensures high electrophoretic mobility and sharp, well-defined bands.
  • Multi-functional: Beyond electrophoresis, its physical properties make it an ideal matrix for creating affinity chromatography beads or for immobilizing enzymes.
  • Clarity and Contrast: Provides a high degree of transparency, facilitating the detection of even low-intensity bands after staining.

Technical specifications

Catalogue number 8045
Brand Condalab
Application PFGE, large DNA fragment separation, and agarose beads
Gel Strength (1.5%) ≥ 1,800 g/cm²
Gelation Temperature (1.5%) 36 ± 1.5 °C
Electroendosmosis (EEO) ≤ 0.12
Storage Temperature 15 – 25 °C (Dry environment)

Available packaging options

8045 500 g plastic bottle
Brand Condalab
Pack size 500 g