1451 BPRM Broth Base (Bacteroides Phage Recovery Medium), Condalab

The medium is designed to provide the strict anaerobic and nutrient-rich conditions required by Bacteroides species. The Peptone Mixture and Meat Extract provide essential nitrogen, amino acids, and vitamins. Yeast Extract serves as a potent source of B-complex vitamins. Glucose acts as the primary fermentable carbohydrate. To ensure successful phage infection and replication, Calcium Chloride and Magnesium Sulfate are included, as divalent cations are essential for the adsorption of phages to the bacterial host cells. Sodium Chloride maintains osmotic balance, and Potassium Phosphate acts as a buffer. The medium is often supplemented with Hemin and Vitamin K1 to further support the fastidious growth of anaerobic Bacteroides.

Advantages

• Optimized Phage Recovery: Specifically formulated to facilitate the attachment and replication of bacteriophages targeting Bacteroides fragilis.
• High Specificity: Facilitates the detection of human-specific fecal indicators, allowing for the differentiation between human and animal contamination in water quality monitoring.
• Robust Anaerobic Support: Provides a nutrient-dense environment that promotes rapid biomass development of anaerobic hosts.
• Divalent Cation Enriched: Contains optimized levels of Calcium and Magnesium to maximize phage plaque formation or recovery rates.
• Standardized Environmental Tool: Used in specialized laboratories for the microbial source tracking (MST) of water pollution.

Technical specifications

Available packaging options