Kupferberg's medium provides a rich nutritional base essential for the metabolic requirements of Trichomonas. The Trypticase (Pancreatic Digest of Casein) provides nitrogen, amino acids, and peptides, while Yeast Extract supplies essential B-complex vitamins and growth factors. Glucose (Dextrose) is included as an energy source for the carbohydrate metabolism of the parasite. Sodium Chloride maintains the osmotic equilibrium. Differentiation and selectivity are achieved through the addition of antibiotics and the indicator Chlorophenol Red. When used with the recommended serum supplement, the medium creates an ideal environment for the multiplication of the trophozoites, allowing for their detection via microscopic examination.
Advantages
- Optimized for Clinical Diagnosis: Specifically engineered to recover Trichomonas vaginalis from complex clinical samples.
- High Growth Support: The combination of Trypticase and Yeast Extract ensures rapid multiplication of the parasites.
- Suppression of Background Flora: When supplemented with antibiotics (like Penicillin and Streptomycin), it effectively prevents the overgrowth of bacteria and fungi.
- Improved Longevity: The formulation maintains the viability of Trichomonas during transport and enrichment phases.
- Clear Visual Monitoring: The presence of Chlorophenol Red helps monitor pH changes during the growth of the culture.
Technical specifications
| Catalogue number |
2029 |
| Brand |
Condalab |
| Application |
Cultivation and detection of Trichomonas vaginalis |
| Typical Composition (g/L) |
Trypticase (20.0); Yeast Extract (1.5); Glucose (1.0); Sodium Chloride (1.0); Chlorophenol Red (0.03) |
| Final pH (at 25 °C) |
6.0 ± 0.2 |
| Preparation |
23.5 g/L (plus Serum/Antibiotics) |
| Storage Temperature |
2 – 25 °C |
Available packaging options
| 2029 |
500 g plastic bottle |
