NZCYM Broth (NutriSelect Plus equivalent) is formulated to provide an abundance of amino acids and growth factors. The name reflects its composition: N-Z-Amine (a pancreatic digest of casein), Z-Amine, Casamino acids, Yeast extract, and Magnesium sulfate. The combination of enzymatic digests of casein and acid-hydrolyzed Casamino acids provides a comprehensive profile of peptides and free amino acids. The Yeast extract supplies essential B-complex vitamins, while Magnesium sulfate ($MgSO_4$) is critical for the adsorption of lambda phage to the E. coli host cells. The inclusion of Sodium chloride maintains the osmotic balance necessary for cell viability during infection.
Advantages
- Optimized for Bacteriophage: Specifically designed to achieve high titers of bacteriophage lambda by providing the necessary ions for viral attachment.
- Maximum Growth Rates: The rich blend of nitrogen sources (NZ-Amine and Casamino acids) ensures the fastest possible doubling times for E. coli.
- Enhanced Protein Expression: Ideal for recombinant protein production where high biomass is required.
- Reliable Performance: Consistent nutrient levels ensure reproducible results in molecular cloning and library screening.
- Versatile Nutrient Profile: Supports the growth of fastidious recombinant strains that might struggle in leaner media like LB.
Technical specifications
| Catalogue number |
1549 |
| Brand |
Condalab |
| Application |
Molecular Biology / Propagation of Bacteriophage Lambda |
| Typical Composition (g/L) |
NZ-Amine (Casein Digest) (10.0); Sodium Chloride (5.0); Casamino Acids (1.0); Yeast Extract (5.0); Magnesium Sulfate anhydrous (0.98) |
| Final pH (at 25 °C) |
7.0 ± 0.2 |
| Preparation |
22.0 g/L |
| Storage Temperature |
2 – 25 °C |
Available packaging options
| 1549 |
500 g plastic bottle |
