Luria Agar, commonly known as Miller’s LB Agar, is a nutritionally rich selective and maintenance medium used for the growth and cultivation of Escherichia coli in molecular biology. It is one of the most widely used agar media for routine cloning, DNA plasmid production, and protein expression.
Luria Agar (Miller’s modification) is based on the original LB (Lysogeny Broth) formulation but contains a higher concentration of Sodium chloride (10.0 g/L) compared to the Lennox formulation (5.0 g/L). This higher salt content is optimized for specific E. coli strains that require a higher osmotic pressure for optimal growth and plasmid stability. The medium provides essential nutrients through Tryptone (amino acids and peptides) and Yeast Extract (B-complex vitamins and trace elements). The addition of bacteriological agar provides a solid surface for colony isolation and the selection of recombinant clones.
Advantages
- Optimized for Miller Strains: Specifically formulated for E. coli strains that thrive in high-salt environments.
- High Colony Contrast: The clear, straw-colored agar allows for easy visualization and picking of colonies, especially during blue/white screening.
- Versatile Selection: Ideal base for supplementation with various antibiotics (e.g., Ampicillin, Kanamycin) for the selection of transformed cells.
- Consistent Performance: Condalab’s rigorous quality control ensures minimal batch-to-batch variation, critical for sensitive molecular experiments.
- Superior Nutrients: High-quality tryptone and yeast extract support rapid cell division and high biomass yields.
Technical specifications
| Catalogue number | 1552 |
| Brand | Condalab |
| Application | Molecular Biology / Cultivation of E. coli |
| Typical Composition (g/L) | Tryptone (10.0); Yeast Extract (5.0); Sodium Chloride (10.0); Bacteriological Agar (15.0) |
| Final pH (at 25 °C) | 7.0 ± 0.2 |
| Preparation | 40.0 g/L |
| Storage Temperature | 2 – 25 °C |
Available packaging options
| 1552 | 500 g plastic bottle |
| Brand | Condalab |
| Pack size | 500 g |