Luria Agar (Miller’s Modification) follows the formulation described by Miller, which is characterized by a Sodium chloride concentration of 10.0 g/L. This is double the salt content of the Lennox formulation, providing the higher osmotic pressure preferred by many E. coli host strains during plasmid amplification and recombinant protein expression. The medium provides high-quality Tryptone as a source of peptides and amino acids, and Yeast Extract for essential B-vitamins and trace elements. The inclusion of bacteriological agar allows for the isolation of individual colonies and the screening of transformants on a solid surface.
Advantages
- Optimized Osmotic Balance: The 10 g/L NaCl concentration is ideal for the growth of many standard laboratory E. coli strains.
- High Biomass Production: Rich nutrient profile supports rapid doubling times and high cell density.
- Versatile Base: Excellent for the addition of antibiotics (e.g., ampicillin, kanamycin, chloramphenicol) for selection purposes.
- Excellent Clarity: The transparency of the agar facilitates easy colony counting and the identification of phenotypic markers.
- Molecular Biology Standard: One of the most widely cited and utilized formulations for cloning and DNA research globally.
Technical specifications
| Catalogue number |
1308 |
| Brand |
Condalab |
| Application |
Molecular Biology / Maintenance of E. coli |
| Typical Composition (g/L) |
Tryptone (10.0); Yeast Extract (5.0); Sodium Chloride (10.0); Bacteriological Agar (15.0) |
| Final pH (at 25 °C) |
7.0 ± 0.2 |
| Preparation |
40.0 g/L |
| Storage Temperature |
2 – 25 °C |
Available packaging options
| 1308 |
500 g plastic bottle |
