1538 RM Base Medium, Condalab

RM Base Medium provides a defined and nutritionally optimized environment for the PL expression system. The PL promoter (from bacteriophage lambda) is a strong, regulated promoter often used for high-level protein production. This medium utilizes Casamino acids as a source of nitrogen and essential amino acids, along with Ammonium chloride. A robust phosphate buffer system (Disodium and Monopotassium phosphate) ensures pH stability during the growth phase. Unlike Induction Base Medium, RM Base Medium is typically used during the growth and maintenance phases to ensure high cell density and genetic stability of the host strains before the induction process is triggered.

Advantages

• Optimized for PL Promoter Strains: Formulated specifically to support the growth of the GI724, GI826, and GI698 E. coli lineage.
• High-Density Growth: Rich in amino acids derived from Casamino acids, promoting rapid doubling times.
• Defined Nutrient Profile: Ensures reproducible results in protein expression experiments by using purified nitrogen sources.
• pH Stability: The dual phosphate buffer system effectively neutralizes metabolic by-products during intense growth.
• Molecular Biology Standard: Essential for maintaining the specific metabolic state required for successful subsequent induction.

Technical specifications

Available packaging options