sLB Agar is a modification of the classic Luria-Bertani medium, optimized for maximum biomass yield. It contains a higher concentration of Tryptone and Yeast Extract than standard LB, providing an abundance of peptides, amino acids, vitamins, and trace elements. This enriched environment allows E. coli to maintain exponential growth for longer periods, resulting in larger colonies and higher yields of DNA or recombinant proteins. The Sodium chloride concentration is balanced to maintain osmotic stability, while the addition of bacteriological agar provides a solid framework for colony isolation and the selection of transformed clones.
Advantages
- Enhanced Growth: Formulated to achieve significantly higher cell densities than standard LB or Miller formulations.
- Optimized for Recombinant Strains: Supports the metabolic demands of cells carrying high-copy-number plasmids or expressing complex proteins.
- Robust Colony Formation: The rich nutrient profile leads to faster colony development, saving time in cloning workflows.
- Ideal for Selection: Provides an excellent base for the addition of antibiotics, ensuring clear differentiation between resistant and sensitive cells.
- High Consistency: Manufactured by Condalab to rigorous standards, ensuring reproducible growth characteristics across different batches.
Technical specifications
| Catalogue number |
1432 |
| Brand |
Condalab |
| Application |
Molecular Biology / High-density E. coli growth |
| Typical Composition (g/L) |
Tryptone (12.0); Yeast Extract (24.0); Sodium Chloride (5.0); Bacteriological Agar (15.0) |
| Final pH (at 25 °C) |
7.0 ± 0.2 |
| Preparation |
45.0 g/L |
| Storage Temperature |
2 – 25 °C |
Available packaging options
| 1432 |
500 g plastic bottle |
