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Y1375 YPD Broth, Merck

Y1375 Merck

YPD Broth (Yeast Peptone Dextrose Broth), part of the NutriSelect Basic product line, is a nutrient-rich non-selective liquid growth medium widely used for the maintenance and propagation of various yeast species, including Saccharomyces cerevisiae and Pichia pastoris.

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YPD Broth is a standard basal medium recommended for routine cultivation and molecular biology studies of yeasts. Its simple yet balanced formulation consists of yeast extract, peptone, and glucose (dextrose). The bacteriological peptone provides essential nitrogenous nutrients and amino acids, while the yeast extract supplies B-complex vitamins and additional growth factors. Glucose serves as the primary carbohydrate and energy source. Due to its high nutrient content, it supports high-density cultures and is ideal for protein expression, molecular cloning, and general yeast genetics research.

Advantages

  • Supports robust growth of most heterotrophic microorganisms and a wide range of yeast species.
  • NutriSelect® Basic quality grade provides a cost-effective powdered format for routine laboratory applications.
  • Consistent formulation ensures reproducible results in molecular biology and industrial fermentation.
  • Easy and quick to prepare with standard laboratory equipment.

Technical specifications

Catalogue number Y1375
Product line NutriSelect® Basic
Synonyms Yeast Peptone Dextrose Broth; YEPD Broth
Form Powder (Beige)
Final pH 6.5 ± 0.2 (25 °C)
Composition (g/L) Bacteriological peptone (20.0); Glucose (20.0); Yeast extract (10.0)
Preparation 50.0 g/L
Storage temperature 15–25 °C (Dry and tightly closed)

Available packaging options

Y1375-250G 250 g poly bottle
Y1375-1KG 1 kg poly bottle
Brand Merck