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146023 Mannitol Salt Agar, Merck

146023 Merck

Mannitol Salt Agar (Heimplate) is a ready-to-use selective and differential culture medium used for the isolation and presumptive identification of Staphylococcus aureus in non-sterile pharmaceuticals, food, and environmental samples.

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Mannitol Salt Agar is a modified version of the selective medium proposed by Chapman (1945). It is specifically formulated to support the growth of staphylococci while inhibiting most other bacteria due to its high sodium chloride concentration (7.5%). The medium differentiates Staphylococcus aureus based on its ability to ferment mannitol, which produces acid and changes the phenol red indicator from pink/red to yellow. This ready-to-use version is designed as a settle plate for long incubation periods, aseptically filled in 90 mm plates to ensure optimal performance in pharmaceutical and industrial microbiology applications.

Advantages

  • Ready-to-use 90 mm settle plates eliminate the need for medium preparation and sterilization.
  • High salt concentration (7.5% NaCl) provides strong selectivity against non-halophilic microorganisms.
  • Clear visual differentiation of mannitol-fermenting S. aureus (yellow colonies with yellow zones).
  • Aseptically filled and designed for long incubation periods (18–72 hours).
  • Compliant with the requirements for testing non-sterile products according to harmonized pharmacopoeias (EP, USP, JP).

Technical specifications

Catalogue number 1.46023
Product line Heimplate™ (Millipore)
Format Ready-to-use settle plate
Plate diameter 90 mm
Filling volume 30 mL
pH Value 7.4 ± 0.2 (H2O, 25 °C)
Suitability Staphylococcus spp.
Storage temperature 15–25 °C

Available packaging options

1.46023.0020 Box of 20 plates
1.46023.0120 Box of 120 plates
Chemical type Microbial Culture Media
Brand Merck