Yeast Nitrogen Base With Amino Acids is a highly-referenced medium containing essential vitamins, inorganic salts, trace elements, and specific amino acids (L-histidine, DL-methionine, and DL-tryptophan). It serves as a satisfactory nitrogen source for general yeast growth when supplemented with a carbohydrate source, typically glucose. The medium is widely used in molecular genetics, the preparation of imaging media, and studies involving synthetic dextrose (SD) medium to evaluate nitrogen source influences.
Advantages
- Comprehensive formulation providing nitrogen, vitamins, trace elements, and salts for robust yeast fermentation.
- Suitable for yeast classification based on the ability to utilize different carbon sources.
- Consistent performance in molecular biology applications, including ZYP-31 media preparation.
- High solubility (67 mg/ml in water) allowing for the preparation of 10x concentrated stock solutions.
Technical specifications
| Catalogue number |
Y1250 |
| Brand |
Sigma-Aldrich |
| Grade |
Molecular Biology |
| Form |
Powder |
| pH |
5.4 ± 0.1 (0.67% solution at 25 °C) |
| Nitrogen Source |
Ammonium sulfate (5.0 g/L) |
| Amino Acids (per L) |
L-histidine (10 mg), DL-methionine (20 mg), DL-tryptophan (20 mg) |
| Storage temperature |
Room temperature (15–25 °C) |
Available packaging options
