YSG Broth is designed to meet the unique physiological requirements of Alicyclobacillus, which thrives in high-temperature, low-pH conditions. Yeast Extract provides the primary source of nitrogen, essential B-complex vitamins, and growth factors. Glucose serves as the main fermentable carbohydrate and energy source. Soluble Starch is included to act as a protective agent, neutralizing metabolic by-products and enhancing the recovery of stressed spores. The medium is adjusted to an acidic pH to mimic the environment of fruit juices, effectively inhibiting most common bacterial contaminants while allowing the target acidophilic bacteria to proliferate.
Advantages
- Targeted Enrichment: Specifically optimized for the recovery of Alicyclobacillus acidoterrestris and related species from fruit concentrates and beverages.
- High Spore Recovery: The addition of starch helps protect and stimulate the germination of heat-stressed spores often found in pasteurized products.
- Standardized Industry Tool: Widely used in the quality control laboratories of the beverage industry to detect potential spoilage before product release.
- Acidic Selectivity: The low pH naturally suppresses the growth of non-acidophilic microflora, reducing the risk of false positives.
- Nutrient-Rich: Provides a robust base that supports rapid biomass development for subsequent molecular or biochemical identification.
Technical specifications
| Catalogue number |
2100 |
| Brand |
Condalab |
| Application |
Cultivation and enrichment of Alicyclobacillus species |
| Typical Composition (g/L) |
Yeast Extract (2.0); Glucose (1.0); Soluble Starch (2.0) |
| Final pH (at 25 °C) |
3.7 ± 0.2 |
| Preparation |
12.0 g/L (adjust pH if necessary after sterilization) |
| Storage Temperature |
2 – 25 °C |
Available packaging options
| 2100-500G |
500 g plastic bottle |
