Vegetable LB Agar (Lennox) replaces traditional animal-derived peptones with a Vegetable Peptone, providing an equivalent profile of nitrogen, amino acids, and peptides. This formulation follows the Lennox modification, which contains a lower concentration of Sodium Chloride (5.0 g/L) compared to the Miller formulation. This lower salt content is often preferred for specific recombinant strains to maintain osmotic balance during plasmid production and to protect salt-sensitive transformation processes. The Yeast Extract provides essential B-vitamins, while the high-quality agar ensures a solid surface for colony isolation and the selection of transformants.
Advantages
- Animal-Free (Non-Animal Origin): Eliminates concerns regarding BSE/TSE contamination, making it ideal for the production of biopharmaceuticals and vaccines.
- Lennox Formulation: The 5 g/L salt concentration is optimized for the growth of specific E. coli strains where higher salt levels may be inhibitory.
- High Compatibility: Performs identically to standard LB Agar (Lennox) in routine cloning, maintenance, and expression experiments.
- Easy Selection: Provides a clear background for blue/white screening and is an excellent base for the addition of antibiotics like ampicillin or kanamycin.
- Strict Quality Control: Manufactured to ensure consistent nutrient levels and growth performance across all batches.
Technical specifications
| Catalogue number |
2039 |
| Brand |
Condalab |
| Application |
Molecular Biology / Animal-Free E. coli Growth |
| Typical Composition (g/L) |
Vegetable Peptone (10.0); Yeast Extract (5.0); Sodium Chloride (5.0); Bacteriological Agar (15.0) |
| Final pH (at 25 °C) |
7.0 ± 0.2 |
| Preparation |
35.0 g/L |
| Storage Temperature |
2 – 25 °C |
Available packaging options
| 2039 |
500 g plastic bottle |
