Terrific Broth is formulated to overcome the nutrient limitations that often lead to early stationary phase in standard media. It contains a high concentration of Tryptone and Yeast Extract, providing an abundance of peptides, amino acids, vitamins, and trace elements. The inclusion of Glycerol as a carbon source, rather than glucose, prevents the rapid accumulation of acetic acid, which can inhibit bacterial growth and degrade recombinant proteins. Furthermore, the medium is heavily buffered with Dipotassium Phosphate and Monopotassium Phosphate to maintain a stable pH during intense metabolic activity, ensuring the health and productivity of the culture over longer incubation periods.
Advantages
- Maximum Biomass: Capable of producing 4 to 10 times higher cell density than standard LB broth.
- Increased Plasmid Yield: The enriched environment supports high-copy-number plasmid replication, resulting in superior DNA recovery.
- Extended Growth Phase: The phosphate buffering system and glycerol carbon source prevent pH shifts that typically limit growth.
- Optimized for Protein Expression: Ideal for the production of recombinant proteins where high biomass is directly correlated with total protein yield.
- High Consistency: Manufactured to strict standards to ensure reproducible growth kinetics and metabolic performance.
Technical specifications
| Catalogue number |
1246 |
| Brand |
Condalab |
| Application |
Molecular Biology / High-yield cultivation of E. coli |
| Typical Composition (g/L) |
Tryptone (12.0); Yeast Extract (24.0); Dipotassium Phosphate (12.5); Monopotassium Phosphate (2.3) |
| Final pH (at 25 °C) |
7.2 ± 0.2 |
| Preparation |
50.8 g/L (plus glycerol) |
| Storage Temperature |
2 – 25 °C |
Available packaging options
| 1246 |
500 g plastic bottle |
