16025 Brilliant Green Bile Lactose Broth (BGLB), Merck

BGLB Broth is specifically formulated to inhibit the growth of Gram-positive bacteria and most non-coliform Gram-negative bacteria. The selectivity is achieved through the synergistic action of ox bile and brilliant green dye. Lactose serves as the fermentable carbohydrate source. Coliforms are characterized by their ability to ferment lactose, producing acid and gas. In this medium, gas production—trapped in an inverted Durham tube—serves as the primary indicator of a positive presumptive coliform test. As part of the NutriSelect® Plus line, this product meets high-quality standards for sensitivity and batch-to-batch reproducibility, ensuring compliance with international testing protocols.

Advantages

• High Selectivity: Effectively suppresses anaerobic gas-formers (like Clostridium perfringens) and Gram-positive organisms that can cause false positives in other media.
• Standardized Reference: Complies with the formulations recommended by APHA, AWWA, and AOAC for water and food analysis.
• NutriSelect® Plus Quality: Premium-grade ingredients ensure optimal recovery of even low numbers of coliforms.
• Clear Interpretation: The combination of the deep green broth and clear gas accumulation in the Durham tube makes results easy to read.
• Broad Applicability: Suitable for testing a wide variety of samples, from drinking water to heavy industrial wastewater.

Interpretation of Results

Technical specifications

Available packaging options