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70191 Mueller Hinton Agar, NutriSelect Plus, Merck

70191 Merck

Mueller Hinton Agar is a specialized microbiological growth medium used primarily for antibiotic susceptibility testing of aerobic and facultatively anaerobic bacteria by the disk diffusion method.

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Mueller Hinton Agar is the standard medium recommended by the Clinical and Laboratory Standards Institute (CLSI) and the FDA for susceptibility testing. Its formulation is specifically designed to be low in sulfonamide, trimethoprim, and tetracycline inhibitors, ensuring accurate and reproducible zones of inhibition. The presence of starch helps to absorb bacterial toxins that might interfere with antibiotic activity, while the loose agar structure facilitates the free diffusion of antimicrobial agents. This NutriSelect® Plus variant is manufactured with high-quality components to ensure batch-to-batch consistency and supports the growth of both fastidious and non-fastidious pathogens, including Neisseria species.

Advantages

  • Standardization: Compliant with CLSI and FDA recommendations for antimicrobial disk diffusion testing.
  • Reproducibility: Provides highly consistent results across different batches.
  • Minimal Interference: Low concentration of known antibiotic inhibitors.
  • Versatility: Supports the growth of a wide range of clinically significant bacteria.
  • Reliability: Starch content neutralizes toxic metabolites, ensuring unhindered antibiotic action.

Technical specifications

Catalogue number 70191
Product name Mueller Hinton Agar, NutriSelect® Plus
Brand Sigma-Aldrich / NutriSelect® Plus
Regulatory compliance According to FDA, NCCLS (CLSI)
Composition (g/l) Casein hydrolysate 17.5; Beef infusion solids 2.0; Starch 1.5; Agar 17.0
Final pH 7.3 ± 0.2 (25 °C)
Form Powder
Storage temperature 10–30 °C
Quality level 200

Available packaging options

Container Type Pack Size
Plastic bottle 100 g
Plastic bottle 500 g
Plastic drum 2.5 kg
Brand Merck