Wort Broth is designed to simulate the nutritional profile of "wort," the malt-derived liquid used in fermentation processes. The Malt Extract is the cornerstone of the medium, providing a dense source of carbon, nitrogen, and essential vitamins, with a high concentration of maltose to drive fungal metabolism. Dextrin and Glycerol (when added) provide additional energy sources and osmotic protection. Ammonium Chloride serves as an inorganic nitrogen source to facilitate protein synthesis and rapid cell multiplication. The naturally low pH of the medium acts as a selective barrier, discouraging the growth of many bacterial species that cannot tolerate acidic conditions, thereby allowing yeasts and molds to proliferate during the enrichment phase.
Advantages
- Fungal Enrichment: Excellent for the recovery and mass cultivation of yeasts and molds from samples with low microbial loads.
- Industry Standard: Widely utilized in brewing for the propagation of culture yeasts and the detection of wild yeast contaminants.
- Selective by Nature: The acidic environment (pH 4.8) minimizes bacterial interference without always requiring the addition of supplemental antibiotics.
- Nutrient-Rich: The malt-based formulation provides a complex array of growth factors that ensure the viability of environmental and industrial fungal strains.
- Versatile Liquid Format: Ideal for use in sterility testing of beverages, syrups, and other liquid food components.
Technical specifications
| Catalogue number |
1444 |
| Brand |
Condalab |
| Application |
Cultivation and enrichment of yeasts and molds |
| Typical Composition (g/L) |
Malt Extract (15.0); Dextrin (2.5); Ammonium Chloride (1.0); Monopotassium Phosphate (1.0); (Glycerol must be added) |
| Final pH (at 25 °C) |
4.8 ± 0.2 |
| Preparation |
33.25 g/L (+ Glycerol) |
| Storage Temperature |
2 – 25 °C |
Available packaging options
| 1444 |
500 g plastic bottle |
