WL Nutrient Agar is designed to support the growth of a wide range of microorganisms, from fermentation yeasts (such as Saccharomyces) to bacterial contaminants. The Casein Peptone and Yeast Extract provide a robust supply of nitrogen, vitamins, and minerals. Glucose is included at a high concentration to serve as the primary energy source for carbon metabolism. The medium is heavily supplemented with various salts—Monopotassium Phosphate, Potassium Chloride, Calcium Chloride, Magnesium Sulfate, Ferric Chloride, and Manganese Sulfate—to ensure that all essential cofactors for enzymatic activity are present. Bromocresol Green is the pH indicator, which allows for the visual differentiation of organisms based on their acid production; as the medium acidifies, the color shifts from blue-green to yellow.
Advantages
- Total Flora Assessment: Ideal for performing total counts of both yeast and bacteria in brewing, winemaking, and distilling.
- Metabolic Differentiation: The indicator Bromocresol Green helps differentiate between different types of yeasts and bacteria based on their colonial morphology and acid-producing capabilities.
- High Nutrient Diversity: Specially formulated with a broad mineral profile to support the growth of fastidious fermentation organisms.
- Quality Control Standard: A staple in the Wallerstein technique for monitoring the health of culture yeast and identifying potential spoilers.
- Clear Results: Provides distinct color changes and colony characteristics that facilitate the identification of wild yeasts and acid-producing bacteria.
Technical specifications
| Catalogue number |
1086 |
| Brand |
Condalab |
| Application |
Cultivation and enumeration of fermentation flora (Total Count) |
| Typical Composition (g/L) |
Glucose (50.0); Casein Peptone (5.0); Yeast Extract (4.0); Monopotassium Phosphate (0.55); Potassium Chloride (0.425); Calcium Chloride (0.125); Magnesium Sulfate (0.125); Ferric Chloride (0.0025); Manganese Sulfate (0.0025); Bromocresol Green (0.022); Bacteriological Agar (20.0) |
| Final pH (at 25 °C) |
5.5 ± 0.2 |
| Preparation |
80.25 g/L |
| Storage Temperature |
2 – 25 °C |
Available packaging options
| 1086 |
500 g plastic bottle |