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1001 Iso-Sensitest Agar, Condalab

1001 Condalab

Iso-Sensitest Agar is a semi-defined medium specifically designed for antimicrobial susceptibility testing (AST). It was developed to overcome the inherent variations found in non-defined media like Mueller Hinton Agar, providing a highly standardized environment that ensures reproducible Minimal Inhibitory Concentration (MIC) results across different laboratories.

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The formulation of Iso-Sensitest Agar is meticulously balanced to minimize the interference of antagonistic components that can affect antibiotic activity. It contains a specialized Peptone Mixture and Casein Hydrolysate to provide a stable source of nitrogen and amino acids. Glucose serves as the primary energy source, while a complex blend of Vitamins and Growth Factors (including Pyridoxine, Thiamine, and Riboflavin) ensures the growth of a wide range of bacteria. To ensure accurate results for sulfonamides and trimethoprim, the levels of Thymine and Thymidine are strictly controlled and kept at a minimum. The medium is also stabilized with a robust mineral and salt profile (including Magnesium Sulfate and Sodium Chloride) to maintain osmotic balance and provide the necessary ions for bacterial metabolism.

Advantages

  • High Reproducibility: The semi-defined nature of the medium reduces batch-to-batch variation, leading to more consistent zones of inhibition.
  • Antagonist-Free: Low levels of para-aminobenzoic acid (PABA) and thymine ensure the high sensitivity of sulfonamide and trimethoprim testing.
  • Universal Growth: Supports the growth of the majority of common clinical pathogens, including Enterobacteriaceae, Staphylococci, and Enterococci.
  • Optimized for AST: The mineral content is carefully adjusted to prevent the interference of divalent cations (Ca²⁺ and Mg²⁺) with aminoglycoside and tetracycline activity.
  • Clarity: The medium is exceptionally clear, making the measurement of inhibition zones simple and accurate.

Technical specifications

Catalogue number 1001
Brand Condalab
Application Antimicrobial Susceptibility Testing (AST)
Typical Composition (g/L) Special Peptone Mix (11.0); Casein Hydrolysate (11.0); Glucose (2.0); Sodium Chloride (3.0); Soluble Starch (1.0); Disodium Phosphate (2.0); Sodium Carbonate (0.6); Growth Factors/Minerals (0.8); Bacteriological Agar (8.0)
Final pH (at 25 °C) 7.4 ± 0.2
Preparation 31.4 g/L
Storage Temperature 2 – 25 °C

Available packaging options

1001 500 g plastic bottle
Brand Condalab
Pack size 500 g