The Amicon® Ultra-0.5 filter (UFC5003) is optimized for ultrafiltration of dilute biological samples containing proteins, antigens, antibodies, enzymes, or nucleic acids. It is particularly effective for concentrating samples from 500 µL down to as little as 15 µL in just 10 to 30 minutes. The vertical membrane design reduces concentration polarization and fouling, ensuring high speed and high sample recovery (typically >90%). These units are also used for desalting, buffer exchange, and removing macromolecular labels or primers from reaction mixes. A unique feature is the reverse spin capability, which allows for consistent and nearly complete recovery of the concentrated retentate.
Advantages
- High Recovery: Retentate recovery is greater than 90% due to the low-binding Ultracel® membrane.
- High Concentration Factor: Can concentrate 500 µL samples 25x to 30x.
- Reverse Spin Recovery: Efficiently collects concentrated protein by centrifuging the filter in a reversed position.
- Fast Processing: Typical spin times range from 10 to 30 minutes for 500 µL samples.
- Versatility: Ideal for everything from desalting and buffer exchange to primer removal.
- Reliable: Physical "dead stop" prevents samples from spinning to dryness.
Technical specifications
| Catalogue number |
UFC5003 |
| Product name |
Amicon® Ultra-0.5 Centrifugal Filter, 3 kDa MWCO |
| Manufacturer |
Merck / Millipore |
| Sample volume |
≤ 500 µL |
| Membrane type |
Ultracel® Regenerated Cellulose |
| MWCO (Cutoff) |
3 kDa (3,000 Dalton) |
| Filtration area |
1 cm² |
| Max RCF |
14,000 x g (fixed-angle rotor) |
| Final concentrate volume |
15 – 20 µL |
| Housing material |
Styrene-butadiene |
Available packaging options
| Catalogue Number |
Pack Size |
| UFC500324 |
Pkg of 24 |
| UFC500396 |
Pkg of 96 |
| UFC5003BK |
Bulk (500 pkg) |
