2194100 Free and Total Copper Color Disc Test Kit, Model CU-6

Free and Total Copper Color Disc Test Set is used to conduct free and total copper analysis in a water sample. The set performs free and total copper determination in the range of 0 - 4 mg/L Cu. Model CU-6 uses Color Disc/ Bicinchoninate, Hydrosulfite reduction method. Free and Total Copper Color Disc Test Kit can be used to conduct 50 free copper tests and 50 total copper tests. The smallest increment is 0.1 Cu. Achieve quick and accurate testing results with the cost-efficient color disc method.

Free and Total Copper Color Disc Test Set includes:

• Free Copper Reagent Powder Pillows (p/n 2182369)
• Hydrosulfite Reagent Powder Pillows (p/n 2118869)
• Copper Color Disc, 0-4 mg/L (p/n 9263300)
• Color Comparator Box (p/n 173200)
• 18 mm Glass Viewing Tubes (p/n 173006)
• Stoppers for 18-mm Glass Tubes and AccuVac Ampuls (p/n 173106)

Procedure for Cu measurement

Before you start take into account the following:

• Undissolved reagent has no effect on test accuracy.
• High concentrations of cyanide prevent color development.
• Subtract the mg/L free copper from the mg/L total dissolved copper, in order to determine the mg/L of complexed copper.

Testing procedure

Fill two tubes to the 5 mL line with sample. Place the first tube into the left opening of the color comparator box. Add Free Copper Reagent Powder Pillow to the second tube, install the stopper on the tube and invert the content of the second tube several times. Wait 2 minutes till the purple color appears as the result of the reaction. Insert the second tube into the color comparator box. Set the color comparator box in front of the light. Rotate the color disc to find the color match. Record the mg/L free copper concentration in the scale window.

In order to conduct the determination of the total dissolved copper, pour the content of Hydrosulfite Reagent Powder Pillow to the second tube. Put the stopper on the second tube and invert to mix. After 2 minutes, install the second tube into the color comparator box. Set the color comparator box in front of the source of light, rotate the color disc to match the color and analyze testing results.

Summary of method

The free copper in the sample reacts with the bicinchoninic acid in the Free Copper Reagent Powder Pillow, resulting in a purple color. The complexed (chelated) copper in the sample reacts with the hydrosulfite reagent, resulting in an additional purple color. The result after adding both reagents is the total amount of dissolved copper. To determine the mg/L of complexed copper, subtract the mg/L free copper from the mg/L total dissolved copper.