This medium is based on the universal Trypticasein Soy Broth formula, which provides a rich supply of nitrogen, vitamins, and amino acids through the combination of Tryptone (pancreatic digest of casein) and Soy Peptone. Glucose acts as the primary energy source, and Dipotassium Phosphate serves as a buffer to maintain a stable pH. The addition of Tween 80 (Polysorbate 80) serves a dual purpose: it acts as a non-ionic surfactant to help emulsify oily or fatty samples, ensuring better contact between the microorganisms and the nutrients, and it also functions as a neutralizer against certain preservatives, such as hexachlorophene and quaternary ammonium compounds, which might otherwise inhibit microbial growth during testing.
Advantages
- Effective Emulsification: Ideal for testing hydrophobic materials, creams, and oil-based cosmetics by ensuring a homogenous suspension.
- Preservative Neutralization: Helps overcome the inhibitory effects of specific antimicrobial agents, preventing false-negative results in sterility or limit tests.
- Universal Growth Support: Supports the proliferation of aerobic bacteria, yeasts, and molds, including fastidious species.
- High Sensitivity: Optimized for the recovery of stressed or sublethally injured organisms in industrial samples.
- Industry Standard: Widely used in environmental monitoring and quality control protocols for the personal care and pharmaceutical sectors.
Technical specifications
| Catalogue number |
2157 |
| Brand |
Condalab |
| Application |
Enrichment of microorganisms in samples with fats, oils, or preservatives |
| Typical Composition (g/L) |
Tryptone (17.0); Soy Peptone (3.0); Sodium Chloride (5.0); Dipotassium Phosphate (2.5); Glucose (2.5); Tween 80 (5.0) |
| Final pH (at 25 °C) |
7.3 ± 0.2 |
| Preparation |
35.0 g/L |
| Storage Temperature |
2 – 25 °C |
Available packaging options
| 2157 |
500 g plastic bottle |
