Agar Test pH 6.0 is designed to facilitate the growth of specific test organisms, typically Bacillus subtilis (BGA), to detect the presence of inhibitory substances. The pH is precisely adjusted to 6.0, which is the optimal acidity for detecting Penicillins and Cephalosporins. If the sample being tested (such as a small disk of meat tissue) contains antibiotic residues, a zone of growth inhibition will form around the sample after incubation. The medium provides a balanced nutrient base of Peptone and Yeast Extract to ensure rapid and uniform growth of the indicator strain, making even small concentrations of residues clearly visible.
Advantages
- Optimized Sensitivity: The pH 6.0 environment is specifically tailored to maximize the detection sensitivity for beta-lactam antibiotics.
- Standardized Food Safety: Follows established guidelines for the detection of inhibitory substances in animal tissues.
- Clear Results: Provides a consistent lawn of growth, allowing for easy measurement of inhibition zones (halos).
- High Purity: Formulated to ensure no natural inhibitory factors are present in the medium itself.
- Versatile: Part of a comprehensive screening system that includes other agar tests at different pH levels (e.g., pH 7.2 and 8.0).
Technical specifications
| Catalogue number |
2022 |
| Brand |
Condalab |
| Application |
Detection of antimicrobial residues (Penicillins) |
| Typical Composition (g/L) |
Peptone (5.0); Yeast Extract (2.0); Meat Extract (1.0); Potassium Dihydrogen Phosphate (2.0); Bacteriological Agar (15.0) |
| Final pH (at 25 °C) |
6.0 ± 0.1 |
| Preparation |
25.0 g/L |
| Storage Temperature |
2 – 25 °C |
Available packaging options
| 2022 |
500 g plastic bottle |
