Actinomycete Isolation Agar is specifically formulated to support the growth of Actinomycetes while suppressing other microorganisms. It contains sodium caseinate and L-asparagine as nitrogen sources, providing essential amino acids. Sodium propionate acts as a substrate for anaerobic fermentation and helps in inhibiting fungal growth. The inclusion of dipotassium phosphate ensures effective buffering to maintain a stable pH. The medium is supplemented with glycerol, which serves as an additional carbon source, and sulfates that provide essential sulfur and metallic ions for metabolic processes.
Advantages
- Highly selective for the isolation of Actinomycetes from complex environmental samples like soil and water.
- Part of the NutriSelect® Plus product line, ensuring standard quality control (Level 200).
- Effective buffering system with dipotassium phosphate to maintain an alkaline pH (8.1), which favors Actinomycete growth.
- Compatible with glycerol supplementation to enhance carbon availability for fastidious strains.
Technical specifications
| Catalogue number |
17117 |
| Brand / Product line |
Sigma-Aldrich / NutriSelect® Plus |
| Quality Level |
200 |
| Form |
Powder (Beige) |
| Final pH |
8.1 ± 0.2 (25 °C) |
| Composition (g/L) |
Agar (15.0); Sodium propionate (4.0); Sodium caseinate (2.0); Dipotassium phosphate (0.5); Magnesium sulfate (0.1); L-Asparagine (0.1); Ferrous sulfate (0.001) |
| Preparation |
22.0 g/L + 5 ml Glycerol |
| Storage temperature |
Below 30 °C (dehydrated powder) |
Available packaging options
| 17117-500G |
500 g plastic bottle |
