This medium is designed to selectively recover members of the Enterobacteriaceae family, even when they are present in small numbers or in a sublethally injured state. The Pancreatic Digest of Gelatin provides essential nitrogen, amino acids, and peptides for growth. Glucose (Dextrose) acts as the fermentable carbohydrate and primary energy source. Selectivity is achieved through the inclusion of Ox Bile (Brilliant Green Bile), which inhibits the growth of Gram-positive bacteria, and Brilliant Green, which further suppresses competitive flora. The medium also features a strong buffering system with Disodium Phosphate and Monopotassium Phosphate to maintain an optimal pH, preventing the rapid accumulation of acid from glucose fermentation which could otherwise inhibit the target organisms.
Advantages
- Pharmacopoeia Compliance: Fully compliant with the harmonized requirements of the EP, USP, and JP for the enrichment of bile-tolerant Gram-negative bacteria.
- High Selectivity: The combination of Ox Bile and Brilliant Green effectively eliminates most Gram-positive contaminants.
- Optimal Recovery: Formulated to support the resuscitation and rapid growth of stressed Enterobacteriaceae commonly found in dry or processed pharmaceutical products.
- Standardized Results: Ensures reproducible performance in quality control laboratories for the testing of raw materials and finished products.
- Clear Indicator: Growth is typically indicated by a change in the color of the medium or visible turbidity, facilitating the transition to confirmatory solid media.
Technical specifications
| Catalogue number |
1202 |
| Brand |
Condalab |
| Application |
Selective enrichment of Enterobacteriaceae (EP/USP) |
| Typical Composition (g/L) |
Pancreatic Digest of Gelatin (10.0); Glucose Monohydrate (5.0); Dehydrated Ox Bile (20.0); Disodium Phosphate Dihydrate (8.0); Monopotassium Phosphate (2.0); Brilliant Green (0.015) |
| Final pH (at 25 °C) |
7.2 ± 0.2 |
| Preparation |
45.01 g/L |
| Storage Temperature |
2 – 25 °C |
Available packaging options
| 1202 |
500 g plastic bottle |