Rogosa SL Agar is designed with a low pH and high salt concentration to create an environment that is selective for lactobacilli. Tryptic Digest of Casein and Yeast Extract provide the essential nitrogen, amino acids, and B-complex vitamins required for growth. The medium is enriched with three fermentable carbohydrates—Glucose, Arabinose, and Saccharose—which serve as diverse energy sources for the different metabolic pathways of various Lactobacillus species. Selectivity is primarily achieved through the high concentration of Glacial Acetic Acid and Sodium Acetate, which combined with a low pH (5.4), effectively inhibit the growth of streptococci, staphylococci, and most other commensal organisms. Manganese Sulfate, Magnesium Sulfate, and Ferrous Sulfate provide essential ions and growth factors that stimulate the proliferation of lactobacilli while maintaining the osmotic balance of the medium.
Advantages
- Extreme Selectivity: Specifically formulated to inhibit the vast majority of competitive bacteria found in clinical and food samples.
- Optimized for Lactobacilli: The triple-sugar blend and specialized mineral profile ensure the recovery of even fastidious Lactobacillus strains.
- Clinical and Food Utility: Ideal for monitoring the microflora of the digestive and vaginal tracts, as well as for testing fermented dairy and food products.
- Clear Identification: Colonies of lactobacilli appear large, white, and opaque, making them easy to distinguish and count.
- Suppression of Molds: The acetate and low pH environment also helps in reducing the growth of many common yeasts and molds.
Technical specifications
| Catalogue number |
1096 |
| Brand |
Condalab |
| Application |
Selective isolation and enumeration of Lactobacillus spp. |
| Typical Composition (g/L) |
Tryptic Digest of Casein (10.0); Yeast Extract (5.0); Glucose (10.0); Arabinose (0.5); Saccharose (0.5); Sodium Acetate (15.0); Ammonium Citrate (2.0); Monopotassium Phosphate (6.0); Magnesium Sulfate (0.57); Manganese Sulfate (0.12); Ferrous Sulfate (0.03); Sorbitan Monooleate (1.0); Bacteriological Agar (15.0) |
| Final pH (at 25 °C) |
5.4 ± 0.2 |
| Preparation |
74.5 g/L (Add 1.32 mL Glacial Acetic Acid) |
| Storage Temperature |
2 – 25 °C |
Available packaging options
| 1096 |
500 g plastic bottle |
