1092 Violet Red Bile Agar with Glucose (VRBG) EP/USP/ISO, Condalab

VRBG Agar is based on the traditional Violet Red Bile Agar formula, but with lactose replaced by Glucose. Since all members of the Enterobacteriaceae family ferment glucose, this medium allows for the detection of the entire group, including non-lactose fermenting pathogens like Salmonella and Shigella. The Pancreatic Digest of Gelatin provides nitrogen and amino acids. Selectivity is achieved through Bile Salts and Crystal Violet, which inhibit Gram-positive bacteria. Neutral Red acts as a pH indicator; when glucose is fermented, the resulting acid production causes the colonies to turn pink to dark red, often surrounded by a reddish precipitation zone of bile salts. To ensure optimal recovery and suppress swarming, the medium is frequently used with an overlayer technique (double layer) to create semi-anaerobic conditions.

Advantages

• Pharmacopoeia and ISO Compliance: Fully meets the requirements of EP/USP/JP and ISO 21528 for the enumeration of Enterobacteriaceae.
• Broad Detection: Detects all glucose-fermenting Gram-negative bacilli, ensuring no Enterobacteriaceae are missed.
• High Selectivity: Effectively suppresses Gram-positive flora, reducing background interference in complex samples.
• Clear Visual Identification: Distinct red/purple colonies with bile precipitation zones allow for easy and accurate counting.
• Standardized Safety Tool: Essential for monitoring hygiene in food production and ensuring the microbial limits of pharmaceutical products.

Technical specifications

Available packaging options