The medium is designed to test for the fermentation of three sugars: Glucose, Lactose, and Sucrose. It contains Beef Extract, Yeast Extract, and Peptone Mixture as sources of nitrogen, vitamins, and minerals. Phenol Red serves as the pH indicator, which turns yellow in the presence of acid (fermentation) and remains red/pink under alkaline conditions. Ferrous Ammonium Sulfate and Sodium Thiosulfate are included to detect the production of Hydrogen Sulfide (H₂S); if H₂S is produced, it reacts with the iron salt to form a black precipitate. The medium is prepared as a "slant," allowing for both aerobic (slant) and anaerobic (butt) growth, providing a comprehensive metabolic profile of the organism in a single tube.
Advantages
- Multi-Parameter Identification: Simultaneously detects glucose fermentation, lactose/sucrose fermentation, gas production, and H₂S production.
- Classic Diagnostic Tool: A cornerstone of the traditional IMViC and enteric identification schemes for clinical and food microbiology.
- High Sensitivity for H₂S: Effectively differentiates H₂S-positive pathogens like Salmonella from H₂S-negative species like Shigella.
- Clear Visual Interpretation: The distinct color changes (Yellow/Red) and blackening provide easy-to-read results.
- Versatile Application: Widely used in the analysis of clinical specimens, water, and food products according to various international standards.
Technical specifications
| Catalogue number |
1046 |
| Brand |
Condalab |
| Application |
Identification of Enterobacteriaceae (Carbohydrate fermentation and H₂S) |
| Typical Composition (g/L) |
Beef Extract (3.0); Yeast Extract (3.0); Peptone Mixture (20.0); Lactose (10.0); Sucrose (10.0); Glucose (1.0); Ferrous Ammonium Sulfate (0.3); Sodium Thiosulfate (0.3); Sodium Chloride (5.0); Phenol Red (0.024); Bacteriological Agar (12.0) |
| Final pH (at 25 °C) |
7.4 ± 0.2 |
| Preparation |
64.52 g/L |
| Storage Temperature |
2 – 25 °C |
Available packaging options
| 1046-500G |
500 g plastic bottle |
