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105910 Maltose monohydrate, Merck

105910 Merck

Maltose monohydrate is a high-purity disaccharide used primarily in microbiology as a carbohydrate source in culture media. It is essential for the identification and differentiation of microorganisms based on their ability to ferment this specific sugar.

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Maltose is a reducing sugar consisting of two glucose molecules joined by an α(1→4) glycosidic bond. In microbiology, it is frequently used to study the nutritional and fermentation profiles of various bacteria and fungi. When added to a base medium (such as Phenol Red Broth), the fermentation of maltose by a microorganism leads to the production of acid, which is visually detected by a change in the pH indicator's color. This product is manufactured to meet the rigorous quality standards required for consistent laboratory performance.

Advantages

  • High Purity: Specified for microbiological applications with controlled levels of heavy metals and consistent hydration.
  • Standardized Performance: Each batch is tested for suitability in microbiology to ensure reliable fermentation results.
  • Easy Visualization: Provides clear, reproducible color transitions when used in media containing pH indicators.
  • Versatile Nutrient: Acts as an essential energy source for the cultivation of a wide range of fastidious and non-fastidious organisms.
  • Long-term Stability: The monohydrate form is stable and easy to handle in a laboratory setting.

Instructions for use

Dissolve the required amount of Maltose monohydrate in distilled or deionized water to reach the desired concentration (typically 5–10 g/L depending on the protocol). It is highly soluble in water (approx. 470 g/L). For use in culture media, add the sugar to a base medium before sterilization. Note: To prevent caramelization or excessive degradation of the sugar, sterilization should be performed by filtration or by autoclaving at a lower temperature/shorter time (e.g., 115 °C for 10–15 minutes) if possible. After inoculation, incubate at the temperature appropriate for the target microorganism.

Interpretation of Results

Reaction Appearance (in Phenol Red Broth) Interpretation
Positive Fermentation Yellow color change Acid produced from maltose
Gas Production Bubble in Durham tube Gas produced during fermentation
Negative Result Remains red/orange No maltose fermentation

Technical specifications

Catalogue number 1.05910
Synonyms 4-O-α-D-Glucopyranosyl-D-glucose, Malt sugar
CAS Number 6363-53-7
Molecular Formula C12H22O11 · H2O
Molar Mass 360.31 g/mol
pH (at 20 °C, 50 g/L) 4.5 – 6.0
Storage Temperature +15 °C to +25 °C

Available packaging options

1.05910.0500 500 g plastic bottle
Brand Merck
Pack size 500 g