Brilliant-Green Bile Lactose Broth (GranuCult® prime) is a granulated dehydrated culture medium containing brilliant green and bile salts to inhibit Gram-positive and many non-target organisms, with lactose as fermentable carbohydrate for coliform detection. The medium complies with ISO 4831, ISO 4832, APHA and FDA-BAM methods for selective enrichment, enumeration and confirmation of Escherichia coli and other fecal coliform organisms.
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105454 GranuCult® prime Brilliant-Green Bile Lactose Broth, Merck (Sigma-Aldrich)
105454
Merck
Brilliant-Green Bile Lactose Broth, catalogue No. 105454, marketed as GranuCult® prime by MilliporeSigma, is a granulated dehydrated culture medium for selective enrichment, enumeration and confirmation of Escherichia coli and other fecal coliform organisms. The medium contains brilliant green and bile as inhibitory agents to suppress Gram-positive and many interfering bacteria, and lactose as a fermentable carbohydrate. It is formulated for use with MPN and related culture techniques in food, water and environmental microbiology testing according to international standards.
Advantages
- Selective enrichment for fecal coliforms including E. coli in complex samples.
- Granulated formulation improves solubility, wettability and handling safety compared to powder media.
- Complies with multiple regulatory methods including ISO 4831, ISO 4832, APHA and FDA-BAM.
- Suitable for reliable detection in food, beverage, water and environmental microbiological quality control.
Technical specifications
| Pack size | 500 g, 5 kg |
| Form | Granulated dehydrated medium |
| Regulatory methods | ISO 4831, ISO 4832, APHA, FDA-BAM |
| Intended use | Selective enrichment, enumeration and confirmation of coliforms / E. coli |
| Typical applications | Food, water, beverage and environmental microbiological analysis |
| Typical pH (prepared) | ≈ 7.2 at 30 °C (40 g/L, after autoclaving) |
| Solubility | 40 g/L |
| Colour | Green |
| Storage conditions | Dry, 15–25 °C; tightly closed |
| Brand | Merck |
