Lyophilized Clostridium perfringens selective supplement containing D-Cycloserine and fluorogenic substrate (MUP), used with TSC Agar base for selective detection and enumeration of Clostridium perfringens.
Clostridium perfringens selective supplement, catalogue No. 100888, is a lyophilized microbiological supplement supplied by MilliporeSigma / Merck used to enhance selectivity of TSC (Tryptose Sulfite Cycloserine) Agar during enumeration of Clostridium perfringens. It contains D-Cycloserine, an antibiotic that inhibits the accompanying bacterial flora, and 4-Methylumbelliferyl phosphate (MUP), a fluorogenic substrate for alkaline and acid phosphatases. The presence of acid phosphatase activity specific to Clostridium perfringens results in release of fluorescent 4-methylumbelliferone detectable under long-wave UV light, aiding in confirmation of C. perfringens colonies.
Advantages
- Selective supplement tailored for Clostridium perfringens enumeration.
- Contains D-Cycloserine to suppress non-target bacterial growth.
- Includes MUP fluorogenic substrate for enhanced detection.
- Lyophilized vial format ensures stability and ease of use.
- Compatible with TSC Agar base for standardized microbiological workflows.
Technical specifications
| Product name | Clostridium perfringens selective supplement |
| Catalogue number | 100888 |
| Product form | Lyophilized supplement in vial |
| Key components | D-Cycloserine; 4-Methylumbelliferyl phosphate (MUP) |
| Intended use | Supplement for TSC Agar to enumerate Clostridium perfringens |
| Technique | Microbiological culture, selective media enrichment |
| Compatible base | TSC (Tryptose Sulfite Cycloserine) Agar |
| Typical applications | Food and beverage microbiology, environmental testing |
| Storage conditions | 2–8 °C |
| Brand | Merck |
