Originally developed by De Man, Rogosa, and Sharpe in 1960, MRS Broth is an enriched medium that provides all the essential nutrients for fastidious lactic acid bacteria. The formulation includes peptones and yeast extract as sources of nitrogen, vitamins, and minerals. Dextrose (glucose) serves as the primary fermentable carbohydrate. The addition of Tween® 80 (polysorbate 80), magnesium sulfate, and manganese sulfate provides essential growth factors and ions. Sodium acetate and ammonium citrate act as selective agents, inhibiting the growth of most accompanying flora, including gram-negative bacteria and many fungi, while supporting the growth of Lactobacilli even at lower pH levels.
Advantages
- Highly nutritious formulation that ensures the growth of even the most fastidious strains of Lactobacillus.
- Part of the NutriSelect® Basic product line, offering reliable quality for routine microbiological testing.
- Effective selective system (citrate and acetate) that suppresses competing environmental bacteria.
- Versatile use for enrichment, cultivation, and enumeration of lactic acid bacteria from food, dairy, and clinical samples.
- Optimized pH (6.2) to favor the proliferation of acidophilic organisms.
Technical specifications
| Catalogue number |
69966 |
| Brand / Product line |
Sigma-Aldrich / NutriSelect® Basic |
| Target organisms |
Lactobacillus spp., Bifidobacterium spp., Lactic acid bacteria |
| Form |
Powder |
| Final pH |
6.2 ± 0.2 (25 °C) |
| Composition (g/L) |
Dextrose (20.0); Peptone (10.0); Beef extract (10.0); Yeast extract (5.0); Sodium acetate (5.0); Ammonium citrate (2.0); Tween® 80 (1.0); Dipotassium phosphate (2.0); Magnesium sulfate (0.1); Manganese sulfate (0.05) |
| Preparation |
52.2 g/L |
| Storage temperature |
Below 30 °C (dehydrated powder) |
Available packaging options
| 69966-500G |
500 g plastic bottle |
