This "Modified" version of the classic Terrific Broth provides a sophisticated nutrient balance to prevent the common pitfalls of high-density bacterial growth. It contains a high concentration of Tryptone and Yeast Extract, providing an abundance of amino acids, vitamins, and trace elements. Unlike standard LB broth, Modified Terrific Broth is heavily buffered with Potassium Phosphates to prevent the acidification of the medium—a result of metabolic byproduct accumulation—which often leads to premature cell death. The formulation is specifically designed to be used with Glycerol as the carbon source instead of Glucose; this prevents catabolite repression and avoids the rapid buildup of acetic acid, allowing the culture to reach a significantly higher biomass.
Advantages
- Enhanced Yields: Produces 4 to 10 times more biomass and recombinant protein than standard Luria-Bertani (LB) media.
- Superior Buffering: The phosphate system maintains a near-neutral pH during the critical late-log and stationary phases.
- Extended Cell Viability: Prevents the "crash" of cultures, allowing for longer induction periods in protein expression protocols.
- Plasmid Stability: Optimized nutrient ratios help maintain the presence of high-copy number plasmids within the host cells.
- Efficient Workflow: Replaces the need for constant pH monitoring or supplemental feeding in many laboratory-scale fermentations.
Technical specifications
| Catalogue number |
1548 |
| Brand |
Condalab |
| Application |
Molecular Biology / High-yield E. coli growth |
| Typical Composition (g/L) |
Tryptone (12.0); Yeast Extract (24.0); Dipotassium Phosphate (9.4); Monopotassium Phosphate (2.2) |
| Final pH (at 25 °C) |
7.2 ± 0.2 |
| Preparation |
47.6 g/L + 4 mL Glycerol |
| Storage Temperature |
2 – 25 °C |
Available packaging options
| 1548 |
500 g plastic bottle |
