This medium is an evolution of traditional phosphate broths, utilizing a high concentration of Tryptose as the sole source of nitrogen and carbon. Tryptose provides a rich mixture of amino acids and peptides that meet the demanding nutritional requirements of fastidious bacteria. Glucose (Dextrose) is included as the primary fermentable carbohydrate and energy source. Sodium Chloride maintains the osmotic equilibrium of the medium. The inclusion of Disodium Phosphate provides a strong buffering capacity, which is essential for neutralizing the acids produced during glucose fermentation, thereby maintaining a stable pH and ensuring the continued viability of the culture. When supplemented with blood or serum, it becomes an even more potent medium for the most demanding organisms.
Advantages
- High Nutrient Density: The large amount of Tryptose ensures rapid and heavy growth of fastidious aerobic and anaerobic bacteria.
- Superior Buffering: The phosphate system prevents rapid acidification, protecting pH-sensitive organisms during extended incubation.
- Cell Culture Applications: Widely used as a supplement in tissue culture media to promote the growth of mammalian cells and facilitate viral replication.
- Versatile Base: Can be easily modified with the addition of agar, blood, or specific inhibitors to serve various diagnostic purposes.
- Reliable Performance: Provides consistent and reproducible results in both clinical diagnostics and industrial fermentation processes.
Technical specifications
| Catalogue number |
1294 |
| Brand |
Condalab |
| Application |
Cultivation of fastidious microorganisms and cell culture supplement |
| Typical Composition (g/L) |
Tryptose (20.0); Glucose (2.0); Sodium Chloride (5.0); Disodium Phosphate (2.5) |
| Final pH (at 25 °C) |
7.3 ± 0.2 |
| Preparation |
29.5 g/L |
| Storage Temperature |
2 – 25 °C |
Available packaging options
| 1294 |
500 g plastic bottle |
