LB Broth (Lennox) is a non-selective liquid medium designed for routine cultivation and maintenance of a wide range of bacteria, including Escherichia coli. It provides balanced nutrition through tryptone and yeast extract and is commonly used for bacterial growth, cloning, plasmid propagation and general microbiological applications.
LB Broth (Lennox), catalogue number 1231, is a dehydrated, non-selective liquid culture medium intended for the cultivation of non-fastidious microorganisms. It is one of the most commonly used media in microbiology and molecular biology laboratories, particularly for the growth of Escherichia coli and related enteric bacteria.
The formulation contains tryptone as a source of peptides and amino acids, yeast extract providing vitamins and growth factors, and sodium chloride to maintain osmotic balance. The Lennox formulation contains a lower concentration of sodium chloride compared to other LB variants, making it suitable for routine bacterial growth and plasmid propagation.
Advantages
- Widely used standard medium for routine bacterial cultivation.
- Supports rapid growth of non-fastidious microorganisms.
- Suitable for microbiology and molecular biology applications.
- Compatible with antibiotic supplementation when required.
Technical specifications
| Pack size | 500 g |
| Medium type | Non-selective liquid culture medium |
| Typical composition (g/L) | Tryptone 10.0; Yeast extract 5.0; Sodium chloride 5.0 |
| Final pH (25 °C) | ≈ 7.0 ± 0.2 |
| Intended use | General cultivation of non-fastidious bacteria |
| Applications | Microbiology, molecular biology, routine laboratory use |
| Storage conditions | 2–25 °C, dry place, tightly closed |
| Chemical type | Microbial Culture Media |
| Brand | Condalab |
| Pack size | 500 g |
