Columbia Broth is characterized by its rich mixture of peptones, providing an exceptional balance of nitrogenous compounds, amino acids, and peptides. The combination of Casein Peptone, Meat Peptone, and Heart Peptone ensures the recovery of even the most demanding pathogens. Yeast Extract and Beef Extract supply essential B-complex vitamins and growth factors, while Glucose acts as an immediate energy source. The medium is supplemented with L-Cysteine to support the growth of organisms with specific sulfur requirements. Magnesium Sulfate and Ferrous Sulfate are included to stimulate bacterial metabolism and enzymatic activity. Sodium Chloride maintains osmotic equilibrium, and the high buffering capacity of the medium helps neutralize metabolic acids produced during growth, ensuring cell viability.
Advantages
- Superior Nutrient Enrichment: Specifically designed to support the rapid growth of fastidious bacteria such as Neisseria, Haemophilus, and Brucella.
- Ideal Blood Culture Base: Frequently used in manual or automated blood culture systems for the detection of bacteremia and septicemia.
- Anaerobic Support: The inclusion of cysteine and the nutrient density make it an excellent base for the cultivation of clinically significant anaerobes.
- High Sensitivity: Capable of recovering microorganisms even when present in very low concentrations in the initial sample.
- Neutralization of Inhibitors: The formulation helps counteract the effects of natural bacterial inhibitors often found in blood samples.
Technical specifications
| Catalogue number |
1229 |
| Brand |
Condalab |
| Application |
Cultivation of fastidious microorganisms / Blood culture |
| Typical Composition (g/L) |
Special Peptone Mix (23.0); Glucose (2.5); Sodium Chloride (5.0); L-Cysteine HCl (0.1); Magnesium Sulfate (0.1); Ferrous Sulfate (0.02); Sodium Carbonate (0.6); Tris-buffer (0.83) |
| Final pH (at 25 °C) |
7.5 ± 0.2 |
| Preparation |
35.0 g/L |
| Storage Temperature |
2 – 25 °C |
Available packaging options
| 1229 |
500 g plastic bottle |
