Dextrose Tryptone Agar with Starch is a general-purpose culture medium used for growth of bacteria and for demonstrating starch hydrolysis by amylase-producing microorganisms. The formulation combines tryptone as a nitrogen source, dextrose for energy, and soluble starch as a substrate for enzymatic degradation.
Dextrose Tryptone Agar with Starch, catalogue number 1146, is a dehydrated culture medium designed for the cultivation of a wide range of microorganisms and for the detection of starch hydrolysis. The medium contains tryptone, which supplies amino acids and nitrogenous compounds, dextrose as a readily fermentable carbohydrate, and soluble starch as a substrate for amylolytic enzymes.
After incubation, starch hydrolysis can be demonstrated by flooding the agar surface with iodine solution. Microorganisms capable of producing amylase hydrolyze starch, resulting in a clear zone around colonies against a dark blue or brown background. This medium is commonly used in microbiological identification procedures and routine laboratory testing.
Advantages
- Supports growth of a broad range of non-fastidious microorganisms.
- Allows detection of amylase activity through starch hydrolysis.
- Simple preparation and straightforward interpretation of results.
- Suitable for routine microbiology, quality control and educational laboratories.
Technical specifications
| Pack size | 500 g |
| Intended use | Cultivation of microorganisms and detection of starch hydrolysis |
| Main components | Tryptone, dextrose, soluble starch, agar |
| Typical incubation | 30–37 °C, 18–48 h (aerobic) |
| Result interpretation | Clear halo around colonies after iodine indicates starch hydrolysis |
| Storage conditions | Store dry, tightly closed, at 2–25 °C |
| Chemical type | Microbial Culture Media |
| Brand | Condalab |
| Pack size | 500 g |