MRS Broth is a type of culture medium used for the cultivation of lactic acid bacteria, particularly species belonging to the genera Lactobacillus, Streptococcus, and Pediococcus from various sources, including food, dairy products, and clinical specimens.
The MRS Broth is composed of a peptone-based medium containing glucose, yeast extract, and polysorbate 80, with an acidic pH. The acidic pH is essential for the selective growth of lactic acid bacteria, as it inhibits the growth of other bacteria.
| ph Value | Solubility |
| 5.5 - 5.9 (50 g/l, H₂O, 30 °C) (after autoclaving) | 50 g/l |
The MRS broth is used for the enrichment and enumeration of lactobacilli, streptococci, and other lactic acid bacteria in food and clinical samples. It supports the growth of these bacteria by providing essential nutrients, such as amino acids, vitamins, and minerals.
The MRS broth is available in a dehydrated form and requires reconstitution with distilled water before use. It is typically sterilized by autoclaving before use.
| Chemical type | Microbial Culture Media |
| Brand | Merck |
| Pack size | 500 g |
