LB Agar (Lennox) is a non-selective, nutrient-rich solid medium widely used for routine cultivation, propagation and maintenance of bacteria, particularly E. coli. The Lennox formulation contains tryptone, yeast extract and sodium chloride at moderate concentration, providing balanced growth conditions for plasmid propagation, cloning, and general microbiological work.
LB Agar (Lennox), catalogue No. 1083, is a dehydrated general-purpose solid culture medium designed for the cultivation and maintenance of non-fastidious microorganisms. It is especially suitable for the growth of Escherichia coli and other Gram-negative bacteria commonly used in molecular biology, genetic engineering and microbiology research.
The Lennox formulation consists of tryptone as a source of peptides and amino acids, yeast extract supplying vitamins and growth factors, sodium chloride to maintain osmotic balance, and bacteriological agar as a solidifying agent. This balanced composition supports robust bacterial growth while avoiding excessive salt concentration.
Advantages
- Widely used standard medium in molecular biology and microbiology laboratories.
- Supports strong and reproducible growth of E. coli and other non-fastidious bacteria.
- Suitable for cloning, plasmid propagation and routine bacterial cultivation.
- Compatible with antibiotic supplementation for selection experiments.
Technical specifications
| Pack size | 500 g |
| Composition (g/L) | Tryptone 10.0; Yeast extract 5.0; Sodium chloride 5.0; Agar 15.0 |
| Final pH (25 °C) | ≈ 7.0 ± 0.2 |
| Intended use | General cultivation and maintenance of non-fastidious bacteria |
| Typical microorganisms | Escherichia coli and other Gram-negative bacteria |
| Application fields | Molecular biology, microbiology, research laboratories |
| Storage conditions | 2–25 °C, dry place, tightly closed |
| Chemical type | Microbial Culture Media |
| Brand | Condalab |
| Pack size | 500 g |