Lactose Broth (LB) is a type of liquid culture medium used in microbiology to grow bacteria, particularly those that are able to ferment lactose. LB contains lactose, which serves as the carbon source for the bacteria, as well as peptone and yeast extract, which provide the necessary nitrogen and growth factors.
ph Value: 6.7 - 7.1 (13 g/l, H₂O, 25 °C)
Store at: +15°C to +25°C
Appearance (color): yellowish
Lactose Broth is commonly used in microbiology research for the cultivation of a variety of bacteria, including Escherichia coli, Salmonella, and Shigella species. It is also used in quality control testing for the detection of bacterial contamination in food, water, and pharmaceutical products.
To prepare LB, the dehydrated medium is typically mixed with water and heated to dissolve the ingredients. The medium is then sterilized by autoclaving and distributed into sterile containers, such as test tubes or flasks, where it can be inoculated with bacteria.
| Chemical type | Microbial Culture Media |
| Brand | Merck |
| Pack size | 500 g |
