GranuCult® prime Triple Sugar Iron Agar is a modification of the classical Kligler’s agar, enriched with 1% sucrose to differentiate Proteus and Hafnia species from Salmonella and Shigella. The medium contains three fermentable sugars in varying concentrations: Glucose (0.1%), Lactose (1.0%), and Sucrose (1.0%). Fermentation of these carbohydrates produces acids that change the color of the pH indicator, phenol red, from red-orange to yellow. Additionally, the medium detects H₂S production; several bacterial species reduce thiosulfate to hydrogen sulfide, which reacts with iron salts to form a black precipitate of iron sulfide. The granulated form of this medium ensures high solubility, safety by reducing dust inhalation, and excellent batch-to-batch reproducibility.
Advantages
- Multifunctional Diagnostics: Simultaneously detects glucose, lactose, and sucrose fermentation, gas production, and H₂S production in a single tube.
- GranuCult® Technology: Superior wettability, flowability, and solubility compared to traditional powders.
- Compliance: Meets the requirements of ISO 6579, ISO 6785, ISO 10272, and various international pharmacopoeias (USP/EP).
- Safe Handling: Significant reduction in fine dust formation, minimizing the risk of inhalation by laboratory staff.
- Clear Results: High-contrast color changes and distinct blackening facilitate unambiguous interpretation.
Technical specifications
| Catalogue number |
103915 |
| Product Line |
GranuCult® prime |
| Form |
Granular |
| Composition (g/L) |
Peptones (20.0); Meat extract (3.0); Yeast extract (3.0); NaCl (5.0); Lactose (10.0); Sucrose (10.0); D(+)Glucose (1.0); Ferric ammonium citrate (0.5); Sodium thiosulfate (0.5); Phenol red (0.024); Agar-agar (12.0) |
| pH (at 25 °C) |
7.4 ± 0.2 (after autoclaving) |
| Solubility |
65 g/L |
| Storage Temperature |
15 – 25 °C |
Reading of Results
| Reaction |
Appearance |
Interpretation |
| Alkaline/Acid (K/A) |
Red slant / Yellow butt |
Only Glucose fermented |
| Acid/Acid (A/A) |
Yellow slant / Yellow butt |
Glucose + Lactose and/or Sucrose fermented |
| Blackening |
Black precipitate in butt |
H₂S production positive |
| Bubbles/Cracks |
Gas formation in agar |
Gas production positive |
Available packaging options
| 1.03915.0500 |
500 g plastic bottle |
